Design and development of the máster in teacher education of secondary education for his first year of implantation

El curso académico 2009/2010 ha sido el primer año de implantación del nuevo máster de profesorado de secundaria en un gran número de universidades españolas. En la Asociación de Profesores e Investigadores en Ciencias Experimentales (APICE) ha surgido la inquietud de indagar en las características de este máster y para ello se ha administrado un cuestionario que ha sido cumplimentado por veinte profesores expertos en la formación de profesores de secundaria. En este trabajo se presentan estos resultados y se aportan las principales fortalezas, debilidades y propuestas de mejora para sucesivos años de implementación

Estudio comparativo de compost de aplicaciones agronómica

It have been studied different types of compost coming from the provinces of Castellón, Granada, Jaén, Las Palmas, Sevilla, and Valencia. The parameters utilized have been: Organic matter, N, P, C/N Humfication degree, salts content, etc .... All the compost studied present a good quality because they get a high level of organic matter so as nutritive elements. The best compost are those of Castellón and Jaen while the compost from Valencia, Sevilla and Granada presents a medium quaUty and the compost of Las Palmas is the worst of the studied compost. The main problem found are a high proportion of salinity. This problem is easy to solve but of course with an increment in the cost.Han sido estudiados diferentes tipos de compost procedentes de las provincias de Castellón, Granada, Jaén, Las Palmas, Sevilla y Valencia. Los parámetros utilizados han sido: Materia orgánica, N, P, C/N Grado de humificación, Salinidad, etc. . . . Todos los compost estudiados son en mayor o menor grado satisfactorios, ya que aportan una elevada cantidad de materia orgánica al suelo y una proporción más que aceptable de nutrientes. Destacan entre las muestras estudiadas las de Castellón y Jaén mientras que las de Valencia, Sevilla y Granada presentan una calidad media, y el compost de Las Palmas es muy inferior. El principal problema encontrado es una elevada salinidad, problema que puede ser fácilmente resuelto, pero con un encarecimiento del producto

High-yield production of short GpppA- and (7Me)GpppA-capped RNAs and HPLC-monitoring of methyltransfer reactions at the guanine-N7 and adenosine-2′O positions

Many eukaryotic and viral mRNAs, in which the first transcribed nucleotide is an adenosine, are decorated with a cap-1 structure, (7Me)G(5′)-ppp(5′)-A(2′OMe). The positive-sense RNA genomes of flaviviruses (Dengue, West Nile virus) for example show strict conservation of the adenosine. We set out to produce GpppA- and (7Me)GpppA-capped RNA oligonucleotides for non-radioactive mRNA cap methyltransferase assays and, in perspective, for studies of enzyme specificity in relation to substrate length as well as for co-crystallization studies. This study reports the use of a bacteriophage T7 DNA primase fragment to synthesize GpppAC(n) and (7Me)GpppAC(n) (1 ≤ n ≤ 9) in a one-step enzymatic reaction, followed by direct on-line cleaning HPLC purification. Optimization studies show that yields could be modulated by DNA template, enzyme and substrate concentration adjustments and longer reaction times. Large-scale synthesis rendered pure (in average 99%) products (1 ≤ n ≤ 7) in quantities of up to 100 nmol starting from 200 nmol cap analog. The capped RNA oligonucleotides were efficient substrates of Dengue virus (nucleoside-2′-O-)-methyltransferase, and human (guanine-N7)-methyltransferase. Methyltransfer reactions were monitored by a non-radioactive, quantitative HPLC assay. Additionally, the produced capped RNAs may serve in biochemical, inhibition and structural studies involving a variety of eukaryotic and viral methyltransferases and guanylyltransferases

Alcohol and Substance Use in the Jewish Community: A Pilot Study

Awareness of addictions in the Jewish community is becoming increasingly prevalent, and yet, a gap exists in the literature regarding addictions in this community. Knowledge about the prevalence of addictions within Jewish communities is limited; some believe that Jews cannot be affected by addictions. To address this gap, a pilot study was conducted to gather preliminary evidence relating to addictions and substance use in the Jewish community. Results indicate that a significant portion of the Jewish community knows someone affected by an addiction and that over 20% have a family history of addiction. Future research needs are discussed

Imunopatologia da esquistossomose mansônica humana: II. Atividade NK e estimulação por antígeno específico

Sixteen S. mansoni infected and untreated patients (5 with recent infection and 11 with chronic disease) were evaluated for their in vitro natural killer (NK) activity against the NK sensitive target K562 cell line. NK levels in 9 out of 11 patients (82%) with chronic disease were significantly lower (mean = 15 ± 6%),compared with patients recently infected (mean = 41 ± 9% p < 0.001) and with the control group (mean = 38 ± 13% p < 0.001). However, both patients and controls NK activity was stimulated by soluble adult worm antigens (SAWA), indicating that NK function even in the chronic stage of the infection is able to respond to the parasite antigens. These results suggest the possibility of NK cell participation as effector mechanism against S. mansoni.Dezesseis doentes infectados e não tratados com S. mansoni (5 com infecção recente e 11 com doença crônica), foram submetidos à avaliação de atividade de células exterminadoras na turais (NK) "in vitro" frente a células alvo de linhagem K562. Os níveis de atividade das céluIas NK em 9 de 11 doentes (827r) com a infecção crônica foram significativamente menores (média = 15 ± 6%) quando comparados aos pacientes com infecção recente (média =41 ± 9%, p < 0,001) e aos indivíduos do grupo controle (média = 38 ± 13%, p < 0,001). Porém, tanto nos doentes como nos controles, a atividade de céluIas NK foi estimulada pelo antígeno solúvel do parasito adulto (SAWA), indicando que as céluIas NK, mesmo na fase crônica da infecção, têm capacidade de responder ao antígeno dos parasitos. Estes resultados sugerem a possível participação das células NK no mecanismo efetor de defesa contra o S. mansoni

Poikilothermia in a 38-year-old Fabry patient

A Fabry patient with poikilothermia is described. Laboratory investigations, neuro-imaging and autonomic function tests did not disclose a cause. Assessment of intra-epidermal nerve fibre density and quantitative sensory testing revealed small fibre neuropathy with a highly impaired cold sensation. We speculate that the poikilothermia is either caused by a vascular lesion in the hypothalamus not visible on MRI or by small fibre neuropathy leading to disturbed body temperature perception and therefore impaired thermoregulation

Constructivism: Defense or a Continual Critical Appraisal – A Response to Gil-Pérez et al.

Abstract. This commentary is a critical appraisal of Gil-Pérez et al.’s (2002) conceptualization of constructivism. It is argued that the following aspects of their presentation are problematic: (a) Although the role of controversy is recognized, the authors implicitly subscribe to a Kuhnian perspective of ‘normal’ science; (b) Authors fail to recognize the importance of von Glasersfeld’s contribution to the understanding of constructivism in science education; (c) The fact that it is not possible to implement a constructivist pedagogy without a constructivist epistemology has been ignored; and (d) Failure to recognize that the metaphor of the ‘student as a developing scientist’ facilitates teaching strategies as students are confronted with alternative/rival/conflicting ideas. Finally, we have shown that constructivism in science education is going through a process of continual critical appraisals

Thalamocortical disconnection affects the somatic marker and social cognition: a case report

Thalamo-cortical connectivity was characterised in a patient with bilateral infarct of the thalami, without evidence of cognitive deficits in everyday life. Patient underwent social and emotional tests, Iowa Gambling Task (IGT), with and without concomitant heart rate variability (HRV) recording and at 3T-MRI to assess thalamo-cortical connectivity. Patient showed impairment at the IGT, in somatic marker, in emotions and theory of mind. MRI documented a bilateral damage of the centromedian-parafascicular complex. Patient's thalamic lesions disconnected brain areas involved in decision-making and autonomic regulation, affecting the somatic marker and resulting in the neuropsychological deficit exhibited by L.C

Dysphagia in multiple system atrophy consensus statement on diagnosis, prognosis and treatment

Multiple system atrophy (MSA) is a neurodegenerative disorder characterized by a combination of autonomic failure plus cerebellar syndrome and/or parkinsonism. Dysphagia is a frequent and disabling symptom in MSA and its occurrence within 5 years of motor onset is an additional diagnostic feature. Dysphagia can lead to aspiration pneumonia, a recognized cause of death in MSA. Guidelines for diagnosis and management of dysphagia in MSA are lacking. An International Consensus Conference among experts with methodological support was convened in Bologna to reach consensus statements for the diagnosis, prognosis, and treatment of dysphagia in MSA. Abnormalities of the oral and pharyngeal phases of swallowing, esophageal dysfunction and aspiration occur in MSA and worsen as the disease progresses. According to the consensus, dysphagia should be investigated through available screening questionnaires and clinical and instrumental assessment (videofluoroscopic study or fiberoptic endoscopic evaluation of swallowing and manometry) at the time of MSA diagnosis and periodically thereafter. There is evidence that dysphagia is associated with poor survival in MSA, however effective treatments for dysphagia are lacking. Compensatory strategies like diet modification, swallowing maneuvers and head postures should be applied and botulinum toxin injection may be effective in specific conditions. Percutaneous endoscopic gastrostomy may be performed when there is a severe risk of malnutrition and pulmonary complications, but its impact on survival is undetermined. Several research gaps and unmet needs for research involving diagnosis, prognosis, and treatment were identified

Candidate Screening of the TRPC3 Gene in Cerebellar Ataxia

The hereditary cerebellar ataxias are a diverse group of neurodegenerative disorders primarily characterised by loss of balance and coordination due to dysfunction of the cerebellum and its associated pathways. Although many genetic mutations causing inherited cerebellar ataxia have been identified, a significant percentage of patients remain whose cause is unknown. The transient receptor potential (TRP) family member TRPC3 is a non-selective cation channel linked to key signalling pathways that are affected in cerebellar ataxia. Furthermore, genetic mouse models of TRPC3 dysfunction display cerebellar ataxia, making the TRPC3 gene an excellent candidate for screening ataxic patients with unknown genetic aetiology. Here, we report a genetic screen for TRPC3 mutations in a cohort of 98 patients with genetically undefined late-onset cerebellar ataxia and further ten patients with undefined episodic ataxia. We identified a number of variants but no causative mutations in TRPC3. Our findings suggest that mutations in TRPC3 do not significantly contribute to the cause of late-onset and episodic human cerebellar ataxias